ISO 12890 pdf download

ISO 12890 pdf download.Water quality — Determination of toxicity to embryos and larvae of freshwater fish — Semi-static method 1 Scope This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio (Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may include an acute toxicity test using Danio rerio to determine the 96-h LC 50 for zebrafish in accordance with ISO 7346-1 , ISO 7346-2 or ISO 7346-3. The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test conditions are made, particularly with regard to the temperature and volume per fish biomass. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. ISO 5667-1 6, Water quality — Sampling — Part 1 6: Guidance on biotesting of samples . ISO 6341 , Water quality — Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) — Acute toxicity test . ISO 7346-1 , Water quality — Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] — Part 1 : Static method . ISO 7346-2, Water quality — Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] — Part 2: Semi-static method . 4 Principle Newly fertilized eggs are exposed to a series of concentrations of the sample to be tested, including controls (concentration 0). For practical reasons, for the purposes of this International Standard eggs are regarded as embryos. The number of surviving and hatched eggs or larvae is recorded daily in all test solutions, which are renewed daily (semi-static method). No food is provided. The standard period of exposure for this test is 1 0 days, but the test may be prolonged up to 1 4 days in order to increase its...