ISO 14718 pdf download

ISO 14718 pdf download.Animal feeding stuffs — Determination of aflatoxin B 1 content of mixed feeding stuffs — Method using high-performance liquid chromatography 1 Scope This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of aflatoxin B 1 content of animal feeding stuffs including those containing citrus pulp. The lower limit of determination is 1 mg/kg. NOTE 1 This International Standard may be applicable for the determination of the aflatoxin B 1 content of a number of raw materials and straight feeding stuffs such as corn gluten, groundnut, palm kernel, copra, citrus pulp, tapioca, soya bean, rice bran, pollard, rape seed, niger seed and cotton seed (see references [1 ] and [2]). These materials were, however, not included in the collaborative testing of the method. NOTE 2 This International Standard may also be applicable for the determination of the content of the sum of the aflatoxins B 1 , B 2 , G 1 and G 2 . However, the method has not been validated for this parameter by collaborative testing. 2 Normative reference The following normative document contains provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subseqent amendments to, or revisions of, this publication do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent edition of the normative document indicated below. For undated references, the latest edition of the normative document referred to applies. Members of IEC and ISO editions maintain registers of currently valid International Standards. ISO 6498:1 998, Animal feeding stuffs — Preparation of test sample . 3 Principle The sample is extracted with chloroform. The extract is filtered and an aliquot portion is purified on a Florisil® 1 ) cartridge and a C 1 8 cartridge. The final separation and determination is achieved by high-performance liquid chromatography (HPLC) using a reverse-phase C 1 8 column, followed by post-column derivatization with iodine or bromine, and fluorescence detection. 4 Reagents and materials Use only reagents of recognized analytical grade.4.4 Control sample. Prepare a control sample of about 2 kg of compound feed with an aflatoxin B 1 content of about 5 mg/kg by combining samples of previous determinations with an aflatoxin B 1 content of about 5 mg/kg. Mix thoroughly. The aflatoxin B 1 content of the control sample should be determined five...