ISO 10504 pdf download

ISO 10504 pdf download.Starch derivatives — Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups — Method using high-performance liquid chromatography 1 Scope This International Standard describes a high-performance liquid chromatographic (HPLC) method for measuring the composition of dextrose solutions, glucose syrups, fructose-containing syrups, hydrogenated glucose syrups, sorbitol, mannitol and maltitol. The constituents are mainly glucose, maltose, maltotriose, fructose, sorbitol, mannitol, maltitol and malto-oligosaccharides. The use of a column packed with cation-exchange resin is essential. 2 Normative references The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of the publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 3696:1 987, Water for analytical laboratory use — Specification and test methods. ISO 5381 :1 983, Starch hydrolysis products — Determination of water content — Modified Karl Fischer method. 3 Principle Saccharide components are separated using high-performance liquid chromatography. Separation is achieved using a cation-exchange column with water as the eluent. The eluted components are detected by means of a differential refractometer, and quantified using an electronic integrator. 4 Reagents All reagents used shall be of recognized analytical reagent grade. 4.1 Special distilled water The water used may be double-distilled of quality grade 1 in accordance with ISO 3696. The most suitable is demineralized water, which prevents contamination of the ion-exchange resin.The water should be filtered by passage through a 0,22 µm filter. Also, it should be degassed by treatment under vacuum, or by use of an in-line degassing unit. The water should be maintained under an inert atmosphere, and preferably at 70 °C to inhibit microbial growth. NOTE Some commercial water-purification devices produce water which is both filtered and degassed. 4.2 Primary standard solutions Prepare solutions (see annex A) containing 1 0 % (or less) dry matter, according to the sensitivity of the refractometer, with compositions as close as possible to that of the samples to be analysed. NOTE Suitable reference materials for the constituents listed in clause 1 can be obtained from established chemical companies. 4.3 Ion-exchange resins, for off-line demineralization of samples. Salts present in the sample will co-elute from the column,...