ISO 11267 pdf download.Soil quality — Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants
1 Scope
This International Standard specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil). This International Standard provides information on how to use this method for testing substances under temperate conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry’s constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 10381-6, Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory ISO 10694, Soil quality — Determination of organic and total carbon after dry combustion (elementary analysis) ISO 10390, Soil quality — Determination of pH ISO 11260, Soil quality — Determination of effective cation exchange capacity and base saturation level using barium chloride solution ISO 11277, Soil quality — Determination of particle size distribution in mineral soil material — Method by sieving and sedimentation ISO 11465, Soil quality — Determination of dry matter and water content on a mass basis — Gravimetric method
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.3.1 contaminant substance or agent present in the soil as a result of human activity 3.2 ECx effect concentration for x % effect concentration (mass fraction) of a test substance that causes x % of an effect on a given end-point within a given exposure period when compared with a control EXAMPLE An EC50 is a concentration estimated to cause an effect on a test end-point in 50 % of an exposed population over a defined exposure period. Note 1 to entry: The ECx is expressed as a percentage of soil to be tested (dry mass) per soil mixture (dry mass). When substances are tested, the ECx is expressed as mass of the test substance per dry mass of soil in milligrams per kilogram. 3.3 ERx effect rate rate of a soil to be tested that causes an x % of an effect on a given end-point within a given exposure period when compared with a control 3.4 limit test single concentration test consisting of at least four replicates each, the soil to be tested without any dilution or the highest concentration of test substance mixed into the control soil and the control 3.5 LOEC lowest observed effect concentration lowest test substance concentration that has a statistically significant effect (probability p < 0,05) Note 1 to entry: In this test, the LOEC is expressed as a mass of test substance per dry mass of the soil to be tested. All test concentrations above the LOEC should usually show an effect that is statistically different from the control. 3.6 LOER lowest observed effect rate lowest rate of a soil to be tested in a control soil at which a statistically significant effect is observed 3.7 NOEC no observed effect concentration highest test substance concentration immediately below the LOEC at which no effect is observed Note 1 to entry: In this test, the concentration corresponding to the NOEC has no statistically significant effect (probability p < 0,05) within a given exposure period when compared with the control.